Correction to “Improved False Discovery Rate Estimation Procedure for Shotgun Proteomics”
نویسندگان
چکیده
منابع مشابه
Improved False Discovery Rate Estimation Procedure for Shotgun Proteomics
Interpreting the potentially vast number of hypotheses generated by a shotgun proteomics experiment requires a valid and accurate procedure for assigning statistical confidence estimates to identified tandem mass spectra. Despite the crucial role such procedures play in most high-throughput proteomics experiments, the scientific literature has not reached a consensus about the best confidence e...
متن کاملFalse Discovery Rate Estimation in Proteomics.
With the advancement in proteomics separation techniques and improvements in mass analyzers, the data generated in a mass-spectrometry based proteomics experiment is rising exponentially. Such voluminous datasets necessitate automated computational tools for high-throughput data analysis and appropriate statistical control. The data is searched using one or more of the several popular database ...
متن کاملImproving false discovery rate estimation
MOTIVATION Recent attempts to account for multiple testing in the analysis of microarray data have focused on controlling the false discovery rate (FDR). However, rigorous control of the FDR at a preselected level is often impractical. Consequently, it has been suggested to use the q-value as an estimate of the proportion of false discoveries among a set of significant findings. However, such a...
متن کاملShotgun Proteomics and Biomarker Discovery
Coupling large-scale sequencing projects with the amino acid sequence information that can be gleaned from tandem mass spectrometry (MS/MS) has made it much easier to analyze complex mixtures of proteins. The limits of this "shotgun" approach, in which the protein mixture is proteolytically digested before separation, can be further expanded by separating the resulting mixture of peptides prior...
متن کاملHow to talk about protein‐level false discovery rates in shotgun proteomics
A frequently sought output from a shotgun proteomics experiment is a list of proteins that we believe to have been present in the analyzed sample before proteolytic digestion. The standard technique to control for errors in such lists is to enforce a preset threshold for the false discovery rate (FDR). Many consider protein-level FDRs a difficult and vague concept, as the measurement entities, ...
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ژورنال
عنوان ژورنال: Journal of Proteome Research
سال: 2016
ISSN: 1535-3893,1535-3907
DOI: 10.1021/acs.jproteome.6b00853